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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Translational models of 3-D organoids and cancer stem cells in gastric cancer research

Fig. 5

Effect of GSK inhibitor in gastric organoid culture. The gastric organoids were fragile and damaged easily during the passaging process. (a) Organoids without GSK inhibitor resulted in apoptotic deaths and damaged the formation of the 3-D structure. (b) The addition of GSK inhibitor (2 µM) supplied in every 2–3 days dramatically reduced and protected the cells-damage and stimulated the cell growth 2-threefold faster than in the control culture of the organoids. Human adenocarcinoma of stomach organoid lines, HCM-BROD-0208-C16 cancer model primary adenocarcinoma of stomach was purchased from American Type Culture Collection (PDM-146™; ATCC, USA) and cultured as its recommended protocol [5] (bright view images, scale bar = 100 μm). (c) The stomach organoids were generated by seeding in 96-well plates and grown 2 days GSK inhibitor (n = 6): Th organoids were incubated with CellTiter-Glo®3D reagent (G9681, Promega, Madison, WL, USA) prior to culture medium addition, and results were acquired to assay (*** P < 0.001)

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