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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Biomimetic nerve guidance conduit containing engineered exosomes of adipose-derived stem cells promotes peripheral nerve regeneration

Fig. 2

In vitro analysis of functional NT-3 mRNA delivery by ExoNT-3. a Schematic illustration of the process of NT-3 mRNA delivery by the exosomes into SCs, which then translated into functional protein. b Fluorescence confocal microscopy images exhibit the endocytosis of the exosomes by SCs. The intracellular distribution of the exosomes were tracked by using DiR label. S100 was used to label SCs and Hoechst was used to counterstain nuclei. PBS was cocultured with SCs as the negative comparison. c Representative western blotting of NT-3 protein expressing in SCs. d qPCR analysis of NT-3 mRNA expression level in SCs. e ELISA was performed to test the secretion of NT-3 in SCs. Data are presented as mean ± SEM in 3 different experiments, *P < 0.05 by one-way ANOVA

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