Fig. 2

ZJU-37 combined with hOPCs effectively promotes OPC survival and myelination in WMI rats. A HI-induced OPC apoptosis was detected by PDGFRα (green) immunofluorescence staining and TUNEL staining (red) in the corpus callosum of each group, scale bar = 20 μm (A). The percent of TUNEL and PDGFRα double-positive cells was analyzed (D). B Myelination ability of ZJU-37 and transplanted hOPCs were assessed by myelin basic protein MBP (red) immunofluorescence staining (B) and quantitative analysis (E). Scale bar = 50 μm. C Representative electron microscopic images of the the myelin sheath in the corpus callosum were shown of each group. Magnification: 20,000×. F The g-ratio was calculated in each group. G Ultrastructural myelin-damage score was calculated in each group. n = 4 for each group. Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001 vs. Ctrl group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. Vehicle group; ^&P < 0.05, ^&&P < 0.01 vs. ZJU-37 group; ^△P < 0.05 vs. hOPCs group