Fig. 5

ZJU-37 suppresses NLRP3 inflammasome activation in astrocytes induced by OGD in vitro. A Representative images of GFAP (red) and NLRP3 (green) immunofluorescence staining in each group of cells cultured in DMEM/F12 medium containing 10% FBS without ZJU-37 and reoxygenated for 24 h of each group. Scale bar = 100 µm. B-C Western blots and quantitative analyses of NLRP3 protein and cleaved caspase-1 (p20) protein expression in primary cultured rat astrocytes of each group. n = 4, triplicates per group. Data are presented as the mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.0001 vs. Ctrl group; ^##P < 0.01, ^###P < 0.001 vs. OGD group; ^&&&P < 0.001 vs. OGD-ZJU37 (5 μM)