Fig. 6

ZJU-37 attenuats OPC apoptosis and dysdifferentiation caused by the OGD-astrocyte-CM. A Flow chart of the experiment with conditioned medium transfer from OGD-injured astrocytes to OPCs in culture. Mixed medium of OGD astrocyte supernatant and DMEM/F12 was used for OPCs group for 24 h. B Annexin V-FITC and PI staining was used to detect apoptosis of primary cultured rat OPCs after incubated for 24 h with the OGD-astrocyte-CM. C Representative immunofluorescence staining of PDGFR-α (green) and MBP (red) counterstained with DAPI (blue) of each group. Scale bar = 100 µm. D Quantitative analysis of apoptosis rate of OPCs of each group. E Quantitative analysis of the percentage of MBP-positive cells of each group. n = 4, triplicates per group. Data are presented as the mean ± SEM. **P < 0.01 vs. Ctrl group; ^#P < 0.05 vs. CM-Vehicle group