Fig. 4

In vitro functional assessments of the SC cocktail-expanded CD34+ cells. a Cell cycle analysis. One of three representative experiments is shown, and the percentage of different phases is summarized in the histogram. b HSC-specific gene expression. Results of qualitative RNA-PCR are shown on the left for one representative of three independent RNA samples analyzed per group, and the results of quantitative PCR are shown in the right. c The morphology and numbers of colony-forming units (CFUs). Morphology (20× objective, scale bar = 50 μm) and colony numbers were recorded on day 14 after cell seeding. All data are shown as means ± SD, n = 3. *p < 0.05, **p < 0.01, ***p < 0.001. BFU-E burstforming unit-erythroid, CFU-GEMM CFU-granulocyte, erythrocyte, macrophage, megakaryocyte, CFU-GM CFU-granulocyte, macrophage, CFU-Mk CFU-megakaryocyte, PC uncultured cord blood HSCs, VC vehicle control