Fig. 3
Establishment of CoCl2-induced retinal degeneration NHP model. A Intraoperative imaging of CoCl2 injection superimposed on fundus autofluorescence (FAF) image. B and C FAF images (1- and 4-month, respectively) showed persistently decreased autofluorescence signal intensities suggestive of RPE damage in the site of CoCl2 injected region within the red dotted lines. The area outlined in white was selected for analysis of morphological changes. D OCT scan showed normal retinal structure outside the CoCl2 injection zone. The red and black arrows indicate the intact ellipsoid zone and RPE/Bruch’s membrane complex, respectively. E1 to E3 tracked OCT line scans from different regions across the CoCl2 injected zone demonstrated persistent outer retinal alterations without recovery of the ellipsoid zone up to 4 months post-CoCl2 injection. Scale bars, 200 μm. F1–F4 Immunofluorescence and H&E staining of retina in un-operated controls show ordered outer nuclear layer and intact photoreceptor outer segments. Scale bar: 10 μm. G1–G4 Immunofluorescence and H&E staining of retina 1 month after CoCl2 damage shows disorganization of the outer nuclear layer and photoreceptor outer segments. Scale bar: 100 μm