Fig. 2

The second passage of Menstrual blood stem cells (MenSCs) culture extracted by ficoll method (a) and by direct culture method (b). MenSCs migration to the decellularized scaffold after 7 days of culture (c). Flowcytometry of MenSCs for CD44 (d), CD 106 (e) and CD 105 (f) markers showed 92.3%, 34.5% and 46.6% overlapping respectively. MenSCs did not detect CD144 as an endothelial cell marker (g) and CD34 as a hematopoietic stem cell marker (h), 5.52% and 7.03% respectively. The MTT assay revealed higher viable cells of MenSCs culture after 10 days when compared to 7 days culture (i)