Fig. 5

Runx2 expression activation in response to HDAC6 inhibition in BMSCs from aged mice. Lentivirus vector loading siRNA against HDAC6 was employed to knockdown HDAC6 expression in BMSCs from aged mice. Then ChIP assays were used to examine HDAC6 (A), acetylated H3K9/K14 (B), acetylated H4K12 (C), and phosphorylated RNA polymerase II (D) occupancy in the Runx2 promoter regions. Quantitative RT-PCR was employed to measure Runx2 expression in BMSCs from aged mice in response to HDAC6 knockdown with and without osteogenic induction (E). ChIP assays were used to examine HDAC6 (F), acetylated H3K9/K14 (G), acetylated H4K12 (H), and phosphorylated RNA polymerase II (I) occupancy in the Runx2 promoter regions in response to tubastatin A treatment. Quantitative RT-PCR was employed to measure Runx2 expression in BMSCs from aged mice in response to tubastatin A treatment with and without osteogenic induction (J). Data are shown as the mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001