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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Serum-free medium and hypoxic preconditioning synergistically enhance the therapeutic effects of mesenchymal stem cells on experimental renal fibrosis

Fig. 1

Hypo-SF-MSCs have ameliorated senescence and an enhanced proliferative ability. a Representative images of normo-10%MSCs, normo-SF-MSCs, and hypo-SF-MSCs at passage 8 (scale bar = 100 µm). b Western blot analysis of Sirt1 and p16INK4a in 10%MSCs, normo-SF-MSCs, and hypo-SF-MSCs. Graphs show densitometric analyses of Sirt1 and p16INK4a expression levels normalized to the GAPDH expression level (n = 5 in each group). c Surviving cells were assessed by a WST-1 assay. Graph shows the absorbance value at each time point (0, 12, and 24 h) (n = 5 in each group). †P < 0.01 versus normo-10%MSCs, ‡P < 0.01 versus normo-SF-MSCs. d Migration activity of MSCs was analyzed using a transwell migration assay. Graph shows the numbers of migrated cells at 10 h (n = 4 in each group). Experiments to evaluate cell proliferation and migration were performed three times and similar results were obtained. Data are means ± S.D. #P < 0.01 (one-way ANOVA followed by Bonferroni’s post-hoc test)

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