Fig. 4From: Serum-free medium and hypoxic preconditioning synergistically enhance the therapeutic effects of mesenchymal stem cells on experimental renal fibrosisHypoxic preconditioning does not affect SF-MSC-induced changes in the macrophage phenotype from M1 to M2. a Western blot analysis of CD163 and CD68 in THP-1 macrophages incubated with conditioned medium from normo-SF-MSCs or hypo-SF-MSCs. Graph shows densitometric analysis of the CD163 expression level normalized to CD68 and GAPDH expression levels (n = 5 in each group). b Expression level of TSG-6 mRNA in normo-10%MSCs, normo-SF-MSCs, and hypo-SF-MSCs (n = 5 in each group). Data are means ± S.D. #P < 0.01 (one-way ANOVA followed by Bonferroni’s post-hoc test)Back to article page