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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Serum-free medium and hypoxic preconditioning synergistically enhance the therapeutic effects of mesenchymal stem cells on experimental renal fibrosis

Fig. 6

Knockdown of HGF in hypo-SF-MSCs reduces the anti-fibrotic effect in TGF-β1-stimulated HK-2 cells. The concentrations of vascular endothelial growth factor (VEGF) (a) and hepatocyte growth factor (HGF) (b) in each conditioned medium were measured by ELISAs (n = 5 in each group). c ELISA analysis showing the VEGF concentration in conditioned medium from SF-MSCs under hypoxia for various durations (n = 5 in each group). d ELISA analysis showing the VEGF concentration in conditioned medium from SF-MSCs cultured exposed to various O2 concentrations (n = 5 in each group). Hypo-SF-MSCs were transfected with HGF siRNA (HGF siRNA/hypo-SF-MSCs) or negative control siRNA (NC siRNA/hypo-SF-MSCs). e The HGF concentration was measured in conditioned medium from NC siRNA/hypo-SF-MSCs and HGF siRNA/hypo-SF-MSCs using an ELISA (n = 5 in each group). f Western blot analysis of p-Smad2 in HK-2 cells stimulated with TGF-β1 for 30 min. Graph shows densitometric analysis of the p-Smad2 expression level normalized to the Smad2 expression level (n = 5 in each group). g Western blot analysis of α-SMA in HK-2 cells stimulated with TGF-β1 for 24 h. Graph shows densitometric analysis of the α-SMA expression level normalized to the α-tubulin expression level (n = 5 in each group). Data are means ± S.D. #P < 0.01, *P < 0.05 (one-way ANOVA followed by Bonferroni’s post-hoc test or Student’s t-test)

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