Fig. 6

Detection of transferred donor CD45⁻/ALDH^br cells in the recipient lung. A Representative images of flow cytometry of lung CD45⁻ cells in donor mCherry-expressing mice. B Flow cytometry of lung CD45⁻ cells in recipient BLM-treated C57BL/6 mice transferred with PBS alone, donor mCherry⁺/CD45⁻/ALDH^dim cells, and donor mCherry⁺/CD45⁻/ALDH^br cells. Recipient lungs were harvested on the day following the cell transfer (day 3, 3 days after BLM-treatment). C Flow cytometry of lung CD45⁻/ALDH^br cells in recipient BLM-treated C57BL/6 mice transferred with PBS alone, donor mCherry⁺/CD45⁻/ALDH^dim cells, and donor mCherry⁺/CD45⁻/ALDH^br cells. Recipient lungs were harvested the next day and 5 days after cell transfer (days 3 and 7). D Histological analysis of mCherry immunostaining in lungs obtained from mCherry-expressing mice (positive control) and wild-type C57BL/6 mice (negative control). E Representative images of mCherry immunostaining in lungs obtained from recipient BLM-treated wild-type C57BL/6 mice transferred with mCherry⁺/CD45⁻/ALDH^br cells