Fig. 3

Administration of TSCs protects from apoptosis in ALI. The lungs from sham mice (a, sham + PBS) and ALI mice that received PBS (b, ALI + PBS), FBs (c, ALI + FB) or TSCs (d, ALI + TSC) were harvested at day 7. a–d Representative images of immunofluorescence staining for TUNEL (green) and DAPI (blue). e Quantitation of TUNEL staining, and the data are presented as mean ± SEM. One-way analysis of variance was performed, from lungs of sham + PBS (n = 3); ALI + PBS (n = 5), ALI + FB (n = 6), and ALI + TSC (n = 5). *P < 0.0001 versus sham + PBS group, ^&P < 0.0001 versus ALI + PBS group, ^$P < 0.001 versus ALI + FB group. f–h Representative images of immunofluorescence co-staining for TUNEL (green) and AQP5 (red, f–h) or SPC (red, i–k), and the lung sections were counterstained with DAPI (blue), on the lung sections from ALI + PBS group. The higher magnification image g and h from the white boxes in f, j and k from the white boxes in i. Scale bars represent 50 µm