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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Fig. 4

TSCs protect MLE-12 from apoptosis induced by BLM in vitro. MLE-12 were treated with PBS (MLE12) or BLM (MLE12 + BLM), and then co-cultured with TSCs (MLE12 + BLM + TSC). a The gating strategy of flow cytometry. Cells were stained for Sca1 to identify TSCs for exclusion (APC, left). MLE-12 cells were incubated at 60 °C for 1 h to induce apoptosis, and then staining for Annexin V (FITC, middle). MLE-12 cells were treated with Triton-100 for 30 min to induce cell death and staining for 7AAD (PerCP-cy5.5, right). b Representative scatter plots of flow cytometry in each group. c Quantitation of flow cytometry assay, and the data are presented as mean ± SEM. One-way analysis of variance was performed. *P < 0.0001 versus MLE12 group. &P < 0.001 versus MLE12 + BLM group. n = 6 for each group

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