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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Fig. 5

TSCs engraft in the lung injured by BLM. The lungs were harvested at day 7 after BLM exposure. af Representative images of the lung, from sham mice (sham + PBS, a and b), ALI mice that received FBs (ALI + FB, c and d) and TSCs (ALI + TSC, e and f). Images showing cells in FITC channel (a, c and e), and merged with nuclear staining for DAPI (blue, b, d and f). White arrowheads point to green cells. g Representative scatter plots of flow cytometry showing the gating strategy: FITC negative (left) and PKH67 positive (right). h FITC+ cells from the lungs of ALI mice that received FBs (ALI + FB, left) and TSCs (ALI + TSC, right). i Bar graph represents quantitation of % green cells in the total lung suspension, and the data is presented as mean ± SEM. Student’s unpaired t test was performed. *P < 0.05, versus ALI + FB group. n = 6 for ALI + FB and n = 5 for ALI + TSC

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