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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Intratracheal transplantation of trophoblast stem cells attenuates acute lung injury in mice

Fig. 6

TSCs differentiate into alveolar epithelial cells in the lung injured by BLM. The lungs were harvested at day 7 after BLM exposure. af Representative images of the lung receiving TSC pre-labeled with PKH67 (green, a and d), and immunofluorescence staining for AQP5 and SPC (red, b and e), and merged images with nuclear staining for DAPI (blue, c and f). White arrowheads point to green+AQP5+ and green+SPC+ cells (ac and df, respectively). White arrows point to green+AQP5and green+SPC cells (ac and df, respectively). Scale bar represents 25 µm. g Representative scatter plots of flow cytometry showing the gating strategy, FITC+ control from TSCs stained with PKH67 (first panel), FITC+ TSCs gated from unstained cells of the lung that received PKH67-pre-labeled TSCs (second panel), APC+ gated from the population of FITC+ cells in the second panel (third panel), and isotype control from the normal lung stained with FITC- and APC-isotypes (fourth panel). h Representative scatter plots of flow cytometry showing that the cells from BLM injured lungs receiving pre-labeled TSCs (FITC) were stained for AQP5 or SPC (APC). The percentage of FITC+ cells in the total lung cell population is shown in the gates (G+, the first and third panels), in which these cells gated for a percentage of FITC+AQP5+ and FITC+SPC+ in total FITC+ cells (second and fourth panels, respectively). i The pie graph shows quantitation of FITC+AQP5+ and FITC+SPC+ in the total FITC+ cells. n = 5 for each group

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