Fig. 8

MEndoT after cardiac injury is FoxC1 dependent. A FoxC1 and CD31 immunostaining in the sham operation and the ischemic hearts in the presence or absence of adFoxC1 transfection (A) and C quantification of FoxC1 expression in endothelial cells. The greatest amount of cardiac endothelial FoxC1⁺ cells was found in the FoxC1 overexpressed ischemic hearts (arrowheads). Scale bar: 50 μm. B Factor VIII immunostaining in these hearts. Scale bar: 100 μm. D, E Number of endothelial cells/high power field (D) and blood vessels (E). F Masson’s Trichrome staining 30 days after myocardial ischemia. G Quantification of scar/LV area. H Cardiac function and structure were assessed by left ventricular fraction shortening (LVFS), and LV remodeling indices including LVEDD (I) and LVEDV (J) 15 days after ischemia. All data are the means ± SEM. p < 0.05: *versus the heart receiving sham-ischemic operation, ^†versus the ischemic hearts without FoxC1 intervention, ^‡versus the ischemic hearts with adFoxC1 transfection (n = 10 per group). Welch ANOVA analyses were performed in C, D, E and I, and one-way ANOVA analysis was used in G, H and J