Fig. 4
From: Targeted gene correction and functional recovery in achondroplasia patient-derived iPSCs
Gene correction of ACH iPSCs by CRISPR-Cas9 and characterization of corrected iPSCs. a Designed sgRNA and ssODNs around the point mutation site. b FACS analysis showed that the ratio of RFP positive cells was 3.6%. c Corrected iPSCs showed normal DNA sequences (Pink arrow indicated a synonymous mutation). d Corrected iPSCs expressed NANOG, OCT4, SOX2, SSEA4 and TRA1-60, and did not express SSEA-1. e Corrected iPSCs stained positive for the AP activity. f Corrected iPSCs showed normal chromosomal number and structure. Bar in all panels: 10 μm