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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp

Fig. 1

Morphological characteristics of hMSC-DP with accumulated passages. a Schema of isolation and culture of hMSC-DP under SE or SF culture conditions. b The morphology of P5, P10 and P20 hMSC-DP under SE or SF culture conditions. Scale bar = 50 μm. c Cell morphology analysis by high-content imaging. hMSC-DP were co-stained with Phalloidin (orange), Hoechst (blue) and WGA (green). Scale bar = 100 μm. n = 3 for each group. d Cellular senescence assay. The percentage of SA-β-gal positive cells was calculated and compared across groups at P5, P10 and P20. The black arrow indicates positive staining of SA-β-gal. Scale bar = 100 μm. n = 3 ~ 5 for each group. SE, serum; SF, serum-free. Data shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant

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