Fig. 2

Proliferation capacity and chromosomal stability of hMSC-DP at different passages. a Colony-forming ability of DPSCs and SHED under SE and SF culture conditions was assessed by CFU-F assays. The numbers of colonies at P5, P10 and P20 were calculated and compared. n = 3 for each group. b Proliferation capacity of three groups of donor-derived DPSCs and three groups of donor-derived SHED was analyzed by population doubling assay. c Proliferation rate of hMSC-DP was assessed by EdU staining. The percentage of EdU-positive cells was calculated at P5, P10 and P20. d Chromosomal stability was assessed by karyotype analysis. The karyotypes of DPSCs and SHED at P5, P10 and P20 were examined. SE, serum; SF, serum-free. Data shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant