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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: CD146 controls the quality of clinical grade mesenchymal stem cells from human dental pulp

Fig. 3

Surface phenotypic profiles and in vitro immunoregulation ability of hMSC-DP at different passages. a MSC surface marker analysis of hMSC-DP at P5, P10 and P20 under SE and SF culture conditions was assessed by flow cytometry. The mean positive rates of CD34, CD45, CD73, CD90, CD105 and CD146 are shown. n = 3 for each group. b The percentage of CD146 positive cells was calculated at P5, P10 and P20 in DPSCs and SHED, independently. n = 3 for each group. c To assess the in vitro immunoregulation ability, hMSC-DP at P5, P10 and P20 were co-cultured with T cells and the percentage of apoptotic T cells was examined by flow cytometry. n = 3 for each group. Data shown as mean (a) or mean ± SEM (b, c). Comparisons with control group: #p < 0.05, ##p < 0.01, ###p < 0.001. NS no significant difference from control group. Comparisons among hMSC-DP at P5, P10 and P20: *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant

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