Fig. 4

Multilineage differentiation of hMSC-DP. a, b Osteogenic capacity of hMSC-DP. The percentage of alizarin red staining compared at P5, P10 and P20 (a). Western blot analysis showed the expression levels of osteogenic markers including Runx2 and ALP (b). n = 3 for each group. c, d Adipogenic capacity of hMSC-DP. The percentage of oil red o positive cells was calculated and analyzed at P5, P10 and P20 (c). Western blot analysis showed the expression levels of adipogenic markers including LPL and PPARγ (d). n = 3 for each group. e Neural differentiation of hMSC-DP. Immunofluorescent staining and western blot analysis showed the expression of neural markers βIII-tubulin and NeuN. Scale bar = 100 μm. SE, serum; SF, serum-free. Data shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. ns not significant