Fig. 4

RNA sequencing of human CD3⁺ T cells showing inhibition of T cell immunity by hWJ-MSCs in vitro. A Venn diagrams showing significantly enriched gene pathways of naïve T cells (T) versus activated T cells (Act.T) group and Act.T versus Act.T + MSCs group by GSEA analysis. B Bar plot showing normalized enrichment score (NES) of significantly changed GSEA pathways of T versus Act.T group and Act.T versus Act.T + MSCs group in GSEA analysis. C GSEA analysis showing IL2-STAT5 and TNFα signaling via NF-κB pathways were enriched in indicated groups (left). Violin plots showing relative mRNA level of genes involved in IL2-STAT5 and TNFα signaling via NF-κB in indicated groups, 2 replicates were shown for each group (right). D GSEA analysis showing glycolysis enrichment in indicated groups (left) and violin plots showing relative mRNA level of genes involved in glycolysis in indicated groups (right). E Summary of number of differentially expressed genes (DEGs) of Act.T versus T group and Act.T + MSCs versus Act.T group. F Venn diagrams of the DEGs among different groups