Fig. 4

PEV preconditioning enhanced viability, migration and attenuated Cocl2-induced hypoxic apoptosis of ADSCs in vitro. After 24 h PEV pre-treatment, Cocl2(700 ng/ml) was used to induce hypoxic apoptosis of ADSCs. Cell apoptosis was evaluated by Live/dead staining and FACS. A Proliferation of ADSCs preconditioned with different concentration of PEVs for different periods of time. B Representative images and quantification of transwell assay. C Calcein-AM/PI staining showed that the dead cells were significantly reduced in the PEV preconditioning groups. The rate of dead cells was expressed as the ratio of dead cells to total cell count. D The apoptosis-related proteins Bax and Bcl2 were evaluated using Western blotting. E FACS showed that Cocl2-induced hypoxic apoptosis rate of ADSC was decreased in PEV preconditioning groups. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01 versus saline; #p < 0.05, ##p < 0.01 versus PEVs (5 µg/ml)