Fig. 5

Suppression of gliosis by transplanted cells. a, b Transcription of genes, Cntfrα, Bmpr, Gfap, and Nfl-b, involved in gliosis in the brain and spinal cord, using qRT-PCR. Fold gene expression was normalized to Gapdh and β-Actin and error bars represent the SEM of triplicate measures (**p ≤ 0.01). Expression of gliosis genes was significantly upregulated in EAE mice but reduced significantly in treated animals. c Immunohistochemical staining of paraffin sections of the brain and spinal cord with antibody astrogliosis marker, GFAP. The inserts (on the right) were magnified and overexposed to enhance contrast. Scale bars represent 50 µm scale bars (magnification: 40 ×). d Quantification of fluorescent intensity of GFAP in the brain and spinal cord sections depicted in c, respectively (**p ≤ 0.01). GFAP levels significantly increased in EAE mice. However, they were reduced considerably in transplanted animals