Fig. 6

Cell transplantation resulted in improvement in myelination of CNS. a Analysis of paraffin sections of the brain and spinal cord stained with LFB to detect myelination. Scale bars represent 50 μm (magnification: 40 ×). b Quantification of LFB stain intensity of paraffin sections shown in a. Symbols, **, &, §, and ¥ indicate significant difference at p ≤ 0.01 between all experimental conditions: healthy control, EAE, EAE + MSCs, and EAE + NSCs. LFB stain intensity was significantly improved in the cortex, cerebellum, and spinal cord in transplanted EAE animals. c, d Transcription of myelination markers, Erk2, Krox-20, Mpz, Mbp, and Mog in the brain and spinal cord. Fold gene expression was normalized to Gapdh and β-Actin and error bars represent the SEM of triplicate measures (**p ≤ 0.01). e Immunohistochemical staining of myelin proteins, MBP and MOG, in the brain and spinal cord paraffin sections. The inserts (on the right) were magnified and overexposed to enhance contrast. Scale bars represent 50 µm scale bars (magnification: 40 ×). f Quantification of fluorescent intensity of myelin proteins in the brain and spinal cord sections depicted in e (**p ≤ 0.01)