Fig. 7

Cell transplantation upregulated neuroprotection markers. a, b Transcription of neuroprotection markers, Bdnf, Tkrb, Ras, Pi3k, Akt, Creb, Fgfr, Raf, Mek1, Mek2, and Erk1, in the brain and spinal cord, respectively, analyzed using qRT-PCR. Fold gene expression was normalized to Gapdh and β-Actin and error bars represent the SEM of triplicate measures (**p ≤ 0.01). All of the tested markers were increased to normal or higher levels in NSC, but not MSC -transplanted animals compared to the healthy controls. c Immunohistochemical staining of paraffin sections of the brain and spinal cord using antibodies against neural proteins, TUJ1 and NESTIN. The inserts (on the right) were magnified and overexposed to enhance contrast. Scale bars represent 50 µm scale bars (magnification: 40 ×). d Quantification of fluorescent intensity of immunostaining in the brain and spinal cord sections depicted in c. (**p ≤ 0.01). Expression of the neural proteins was more significantly increased in transplanted NSCs than MSCs in EAE animals