Fig. 2

Microscopic and immunostaining photographs of the OMEC sheet from Patient 1. A Cell aggregates/microspheres in the cell sheet can be seen under a surgical microscope shortly before transplantation. B, C Phase contrast microscopic view of the confluent cell sheet in areas with few microspheres (B) and with abundant microspheres (C). D–F Immunoconfocal microscopy for keratin 3 (D, green; nuclei counterstained with PI), p75^NTR (E, green), and p63 (F, red; nuclei counterstained with DAPI) in areas with few microspheres. G–I Staining for keratin 3 (G, green), p75^NTR (H, green), and p63 (I, red) in areas with abundant microspheres. Note that keratin 3 staining is negative within the microspheres, while signals for p75^NTR and p63 are enriched in the structures