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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Human bone marrow-derived mesenchymal stromal cells cultured in serum-free media demonstrate enhanced antifibrotic abilities via prolonged survival and robust regulatory T cell induction in murine bleomycin-induced pulmonary fibrosis

Fig. 2

Engraftment of administered MSCs in murine lungs. A MSCs were labeled with CellTracker CM-DiI before injection. DiI-labeled MSCs were detected as the positive fraction in the phycoerythrin (PE) channel using flow cytometry. B Fluorescence microscopy images showing DiI-labeled MSCs with red fluorescence. C MSCs engrafted in murine lungs were detected using flow cytometry on the day after intravenous SF-MSC administration (i.e., at 5 days after BLM OA). D Confirmation of engrafted human-derived MSCs in murine lung tissues using anti-human nuclear antibody staining (red arrows). E At 1, 3, or 10 days after DiI-labeled SF-MSC injection (i.e., at 5, 7, or 14 days after BLM OA), the percentages of engrafted SF-MSCs in the lungs were measured (n = 3 per group). ***P < 0.005. ****P < 0.001. F The percentages of SF-MSCs or S-MSCs engrafted in murine lungs on the day after DiI-labeled MSC injection (n = 3 per group). ****P < 0.001. G The percentages of SF-MSCs engrafted in the murine thymus, spleen, or lung on the day after DiI-labeled SF-MSC injection. The mice in the PBS group were administered only 100 µL of PBS (n = 3 per group). Data are presented as means ± SD

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Stem Cell Research & Therapy

ISSN: 1757-6512