Fig. 3

Sal-B promotes the survival rate of fat transplantation in a nude mouse Coleman fat graft model. (a) Macroscopic views of harvested tissue in the control and Sal-B-treated groups, which indicated the relative size of fat grafts at different time points (n = 5 per time point). (b) Images of H&E-stained tissue sections from the control group and Sal-B-treated group at different time points (scale bar = 200 μm). (c) Restoration of subcutaneous fat tissue in the Sal-B-treated group was confirmed by immunofluorescence staining of perilipin (green). DAPI (blue) staining of nuclei (scale bar = 200 μm). (d) Quantification of H&E Staining: cell integrity, tissue inflammation, presence of cysts/vacuoles, and the extent of fibrosis. (e) Quantification of immunofluorescence staining of perilipin, the level of the living adipocytes was measured by mean fluorescence intensity of perilipin (IOD/area). The data represent the mean ± SD. * P < 0.05, ** P < 0.01. SD, standard deviation