Fig. 2

Principle of MSOT: a A handheld optoacoustic imaging system uses a light source to generate optoacoustic signals. b Optoacoustic responses after irradiating the target tissue with multiple wavelengths are collected and undergoes image reconstruction. c Spectral unmixing algorithms identify and differentiate the spectral contributions of endogenous photo absorbing molecules in the target tissue (e.g. oxy- or deoxygenated haemoglobin) from exogenous probes (e.g. IR Dye 800). d Separate images corresponding to each endogenous and exogenous chromophore is analyzed. Adapted with permission from [19]. Copyright \(\copyright\) 2017, Royal Society of Chemistry