Fig. 1

IL-1β is widely elevated in the spleen, prostate, and bloodstream of NOD-EAP mice. A HE staining showed inflammation and the structure of the prostate in EAP mice and control mice (bar = 200 μm). Inflammation score and weight in the control group and EAP group. B CD11b⁺ cells of the prostate in EAP mice and control mice (bar = 50 μm). C Hyperalgesia test to detect pelvic pain in EAP mice by von Frey filaments (n = 6 per group). D IL-1β mRNA expression in the spleen of EAP mice and control mice by RT-qPCR (n = 6 per group). E IL-1β mRNA expression in the prostate of CP/CPPS mice and control mice by RT-qPCR. F IL-1β protein expression in the spleen of CP/CPPS mice and control mice by Western blotting (n = 6 per group). G IL-1β protein expression in the prostate of CP/CPPS mice and control mice by Western blotting. H IL-1β protein expression in the bloodstream of CP/CPPS mice and control mice by Legendplex™ bead-based immunoassays (n = 6 per group). Error bars indicate SD. *p < 0.05, **p < 0.01, ***p < 0.001