Fig. 5

Macrophages changed from a proinflammatory phenotype to an anti-inflammatory phenotype in the prostate. A Proportion of CD206 and Arg1 in CD45 + F4/80 + macrophages in the prostates of the saline, MSC, and IL-1β-primed MSC groups by flow cytometry. Proportion of CD86 and iNOS in CD45 + F4/80 + macrophages in the prostates of the saline, MSC, and IL-1β-primed MSC groups determined by flow cytometry (n = 6 per group). B Proportion of CD206⁺ cells and CD86⁺ cells in the prostates of the saline, MSC, and IL-1β-primed MSC groups by IF (n = 6 per group, bar = 50 μm). C Proportion of CD11b⁺ cells in the MSC and IL-1β-primed MSC groups by IF (n = 6 per group, bar = 50 μm). D The expression of anti-inflammatory genes in in situ macrophages in the saline, MSC, and IL-1β-primed MSC groups, as determined by RT-qPCR; the expression of proinflammatory genes in in situ macrophages in the saline, MSC, and IL-1β-primed MSC groups, as determined by RT-qPCR (n = 6 per group). Error bars indicate SD. *p < 0.05, **p < 0.01, ***p < 0.001