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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Knockdown of SERPINB2 enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells via activation of the Wnt/β-catenin signalling pathway

Fig. 4

SERPINB2 gene knockdown promoted the expression of COL1A1 and RUNX2 of hBMSCs, meanwhile promoted mineralization and ALP activity of hBMSCs. a–d Immunofluorescence staining of RUNX2 and COL1A1 proteins. On day 3 of osteogenic differentiation, RUNX2 and COL1A1 (red) increased with SERPINB2 knockdown. Cell nuclei were counter-stained with DAPI (blue). Scale bars, 100 um. e–g SERPINB2 knockdown promoted hBMSCs mineralization and ALP activity. For calcium deposits level detecting, hBMSCs were cultured with osteogenic differentiation medium for 15 days. For ALP activity detecting, hBMSCs were cultured with osteogenic differentiation medium for 6 days. Data are expressed as mean ± SD. Assays were performed in triplicates. *P < 0.05; **P < 0.01; ***P < 0.001 compared with the control group

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