Fig. 3

UC-MSCs up-regulated B10 and produced functional factors after co-cultured with B cells in vitro. a Representative FACS images of CD19⁺IL-10⁺ (B10 cells), b percentages of B10 in B cells after co-cultured with MSCs directly or indirectly. c The mRNA of IL-6, IL-10, TGF-β1 and IDO expressed by MSCs co-cultured with or without B cells was analyzed by real-time PCR. d TGF-β1 in supernatant were significantly increased after co-culture. Data are mean ± SD. B cells (n = 6), B cells + MSCs, B cells and MSCs were co-cultured directly (n = 6), B cells + MSCs (transwell), B cells and MSCs were co-cultured indirectly (n = 6). Data are mean ± SD. *p < 0.05, **p < 0.01