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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Transplantation of mesenchymal stem cells ameliorates systemic lupus erythematosus and upregulates B10 cells through TGF-β1

Fig. 4

Knockdown of TGF-β1 by shRNA abolished the upregulation of B10 cell by MSCs. a Fluorescence microscope showed MSCs infected by lentivirus carrying TGF-β1 shRNA and Control shRNA for 48 h. TGF-β1 interference efficiency was determined by real-time PCR (b) and ELISA (c). d Representative FACS images of B10 cells and e percentages of B10 in B cells after co-cultured with MSCs low expression of TGF-β1. Data are mean ± SD. B cells (n = 6), B cells + MSCs-shRNA-Con, B cells co-cultured with MSCs infected by control lentivirus (n = 6), B cells + MSCs-shRNA-TGF-β1, B cells co-cultured with MSCs infected by lentivirus with TGF-β1 shRNA (n = 6). *p < 0.05, **p < 0.01

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Stem Cell Research & Therapy

ISSN: 1757-6512