Fig. 3

Adipogenic differentiation capacity. A Representative image of Oil Red O staining (ORO), scale bar 200 µm, inset = undifferentiated ASCs, larger picture = ASCs after 14 days of adipogenic differentiation, B quantified ORO staining (QORO) and lipid droplet (LD) area/nuclei (µm²), C relative gene expression of AP2 and D PPARγ. Quantitative data presented as the average ± SD. BM = HS medium, AM = adipogenic medium. Statistical analyses were performed using multilevel mixed-effects regression model test for the nested data structure for QORO and Wilcoxon signed-rank test for relative gene expression of AP2 and PPARγ. Statistical significance: * = p < 0.05. All quantitative data are presented as the mean ± SD