Fig. 6

FNDC5-MSCs‐Exo reduced the inflammation by upregulating Nrf2/HO-1 Axis. a Representative Immunostaining of intracellular Nrf2 (scale bars = 20 μm). After 24 h treatment with LPS, the intracellular Nrf2 was detected by immunostaining with specific antibody, whereas the nuclei were detected by DAPI staining. b Western blot analysis of HO-1 under different conditions. c Representative semi-quantification analysis of HO-1. d Nuclear proteins were collected and extracted to measure NF‐κB p65. e Histone3 were used as normalized cytoplasmic and nuclear protein. The secretions of TNF‐α (f), IL‐6 (g) and IL-1β (h) were detected by ELISA. Representative relative mRNA level of CXCL10 (i) and Arg 1 (j). Data are expressed as the means ± SEM; n = 5; *P < 0.05