Fig. 1
Mesenchymal stem cells induced from hiPSCs expressed MSC markers and retained the potential to differentiate into adipocytes, osteocytes, and chondrocytes in vitro. A Representative flow cytometry profiles of iMSCs (454E2), iMSCs (409B2), and BM-MSCs. iMSCs expressed the mesenchymal stem cell markers CD73 and CD105 but not the hematopoietic markers CD34 and CD45. Expression of CD105 was weak on iMSCs compared with BM-MSCs. B iMSCs and BM-MSCs were induced to differentiate into three mesenchymal lineages: adipocytes, osteocytes, and chondrocytes. Following differentiation, the cells were stained by immunocytochemistry for FABP4 (adipogenic marker, red), osteocalcin (osteogenic marker, green), or aggrecan (chondrogenic marker, red). Scale bar, 100 µm. C The cells were also stained with Oil red O to detect adipogenesis, Alizarin red to detect osteogenesis, and Alcian blue as an indicator of chondrogenesis. Scale bar, 100 µm. D iMSCs and BM-MSCs were seeded at a density of 1 × 105 cells/100 mm petri dish in 10% FBS/DMEM. After 14 days of culture, the cells were stained with 0.05% crystal violet. E MTT assay of iMSCs and BM-MSCs. The experiments were repeated three times with the same tendency. Means ± SDs. Student’s t-test