Fig. 2

Human iMSCs and BM-MSCs promoted differentiation of human myogenic cells in a Transwell coculture system. A Experimental design. Human MSCs (iMSCs or BM-MSCs) (upper well) were cocultured with human myogenic cell lines: Hu5/KD3 cells, adult primary myoblasts (hSKMM), or hiPSC-derived muscle progenitors (lower well) in a Transwell system. B Representative images of myotubes formed by human myogenic cells after 7 days in culture. C Fusion index after 1 week of coculture with human MSCs. The cells were stained with MyHC (red), myogenin (green), and DAPI (nuclei, blue). The fusion index was calculated as the percentage of all nuclei that are myogenin-positive and within MyHC-positive myotubes. Scale bar, 100 μm. More than 30 fields from each sample (3 wells/group) were analyzed. Sidak’s analysis