Fig. 5

Integrin αVβ3 activation induced by CTS regulates focal adhesion-related proteins and phosphorylation of FAK to promote early stage of osteogenic differentiation. a Protein expression of talin-1, FAK and vinculin under CTS for 4 d and 7 d. b mRNA expression of talin-1, FAK and vinculin under CTS for 4 d and 7 d. c Protein expression of talin-1, FAK and vinculin under CTS in the GM and RGDyk groups. d mRNA expression of talin-1, FAK and vinculin under CTS in the GM and RGDyk groups. e Protein expression of talin-1, FAK and vinculin under CTS in the GM, NC, shRNA-integrin αV and shRNA-integrin β3 groups. f mRNA expression of talin-1, FAK and vinculin under CTS in the GM, NC, shRNA-integrin αV and shRNA-integrin β3 groups. g Immunofluorescence of vinculin under CTS in the GM, CTS, NC, shRNA-integrin αV and shRNA-integrin β3 groups for 7 d. Scale bar, 50 μm. h Phosphorylation level of FAK under CTS for 7 d. i Phosphorylation levels of FAK in the GM, NC, shRNA-integrin αV and shRNA-integrin β3 groups under CTS. j Protein expression of ALP and RUNX2 in the CTS and Y15 groups under CTS