Fig. 2

Longitudinal clonal tracking in hu-BLT mice: a Area plots show clonal repopulation in whole blood over time from week 13–19. Each colored band is a unique VIS clone and thickness of the band corresponds to frequency of the VIS clone. Dashed black line separates mCherry-H5 VIS clones (below) and EGFP-WT VIS clones (above). b Line plots show changes over time in the total frequency of mCherry-H1 VIS clones (solid red line) and total frequency of EGFP-WT VIS clones (solid green line), as well as percentages of mCherry + cells (dashed red line) and EGFP + cells (dashed green line). c Heatmaps showing percentage change in shared clones between two timepoints. Digits inside white tiles on the diagonal show number of VIS detected at each timepoint. Colors of each heatmap tile correspond to percentage of clones shared, and color key is provided on the right. Digits in each tile show the number of VIS shared between two timepoints. d Renyi’s diversity profiles evaluated using raw count data from two replicates at each timepoint and by varying value of alpha. Renyi’s diversity profiles are arranged with highest diversity at the top to lowest at the bottom. Topmost curve with no overlap or intersection with any other curve has the highest overall diversity. Diversity of curves that overlap or intersect is undefined. e Line plot showing contribution of highest contributing clone at different timepoints. Values reported are exp(H(alpha)) at alpha = ∞