Fig. 5

Epigenetic determinants of vector integration. a Profile plots and heatmap for 10 chromatin features and input sample in active and inactive VIS-proximal genes in uncultured FL-HSPC. Profile plots show mean score for active (blue line) and inactive (red line) proximal genes. Score is calculated from normalized read count (RPKM) for each sample. Each row in heatmap shows expression level of 10 chromatin features in proximal genes from TSS to TES with 2 Kb flanks upstream and downstream. Color scale key shows range of normalized expression. b Profile plots and heatmaps for 10 chromatin features in region flanking ± 1 Kb of each VIS. Profile plots showing mean scores over ± 1 Kb region flanking VIS. Each row in the heatmap shows the expression level of 10 chromatin features in regions flanking ± 1 Kb of VIS. Individual color scale key shows the range of normalized expression for corresponding features. c) Bar plots show number of VIS within ± 1 Kb of enriched region (peak) of different chromatin features. VIS clones and random IS are binned by absolute distance in base pairs (bp) between the enriched region and IS. Bars show number of VIS in each bin. Top panel shows binning for all VIS, middle panel shows top 10 VIS clones, and bottom panel shows random IS falling within ± 1 Kb of enriched region