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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: sTNFRII-Fc modification protects human UC-MSCs against apoptosis/autophagy induced by TNF-α and enhances their efficacy in alleviating inflammatory arthritis

Fig. 4

Transplantation of sTNFRII-MSC regulates the T and B cell subsets balance and ankle cartilage homeostasis in CIA mice. ae sTNFRII-MSC regulates T cell subsets balance in CIA mice. sTNFRII-MSC transplantation inhibited the percentage of pathogenic Th1 (a), Th17 (c) and Tfh (e) cells but increased the percentage of regulatory Th2 (b) and Tregs (d) in the spleens of CIA mice (n = 8). f, g sTNFRII-MSC regulates B cell subsets balance in CIA mice (n = 8). sTNFRII-MSC transplantation inhibited the percentage of pathogenic plasma cells (f) but increased the percentage of regulatory Breg (g) in the spleens of CIA mice. h Immunohistochemical staining of collagen II, ADAMTS-5, MMP-13 and TIMP-1 in cartilage of ankle joints from normal, CIA mice and mice treated with sTNFRII-MSC. Scale bars: 100 μm. il Immunopositive cells were counted individually in three different areas (n = 4). The number of positively stained cells for collagen II (i), ADAMTS-5 (j), MMP-13 (k) and TIMP-1 (l) was averaged. The proportion of immunopositive cells was determined by dividing the number of positive cells by the total number of cells within the cartilage field. m The ratio of MMP-13/TIMP-1 in cartilage of ankle joints from CIA mice was analyzed. Data are presented as the mean ± SD values. *p < 0.05, **p < 0.01, ***p < 0.001

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