Fig. 6

Detection of sTNFRII-MSC in vivo. a Time-course analysis of sTNFRII-MSC migrated to the spleen of CIA mice after transplantation. Detection of EGFP (green) expression and immunofluorescence staining for anti-human CD105 (red) were conducted in the spleen at 1, 3, 7, and 14 days after sTNFRII-MSC implantation. Scale bars: 200 μm. b The number of GFP⁺CD105⁺ cells was counted in the spleen on each of these days after sTNFRII-MSC implantation (n = 5). c Comparison of the numbers of retained GFP⁺CD105⁺ sTNFRII-MSC and EGFP-MSC in the spleen of CIA mice at 14 days after implantation. Scale bars: 200 μm. d Analysis of the numbers of GFP⁺CD105⁺ cells in the spleen of CIA mice at 14 days after implantation (n = 5). e Time-course analysis of sTNFRII-MSC migrated to the ankle joints of CIA mice after transplantation. Detection of EGFP (green) expression and immunofluorescence staining for anti-human CD105 (red) were conducted in the ankle joints at 1, 3, 7, and 14 days after sTNFRII-MSC implantation (n = 5). Scale bars: 200 μm. f Laser confocal was used to detect apoptosis of sTNFRII-MSC migrated into spleen of CIA mice at 14 days after implantation. The expression of cleaved caspase 3 (purple) was determined, and GFP (green) was used to locate migrated sTNFRII-MSC and EGFP-MSC. Scale bars: 25 μm. g Laser confocal was used to detect apoptosis of sTNFRII-MSC migrated into spleen of CIA mice at 14 days after implantation. The expression of LC3B-II (red) was determined, and GFP (green) was used to locate migrated sTNFRII-MSC and EGFP-MSC. Scale bars: 25 μm. Data are presented as the mean ± SD values. *p < 0.05, **p < 0.01