Fig. 8

sTNFRII-Fc modification improves chondrogenic differentiation of MSCs following TNF-α stimulation. a Cells were treated with TNF-α, followed by assaying for chondrogenic differentiation. Alcian Blue staining was used to evaluate the chondrogenic capacity of sTNFRII-MSC. b The absorbance of eluent from (a) was determined at 600 nm for semiquantitation (n = 5). c Cells treated with/without TNF-α, and the expression levels of AGG and SOX-9 were assessed by western blot. d, e Densitometry quantification of western blot from (c). The intensity of each was normalized to β-actin intensity (n = 3). Data are presented as the mean ± SD values. *p < 0.05, **p < 0.01