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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Endometrial mesenchymal stem/stromal cells: The Enigma to code messages for generation of functionally active regulatory T cells

Fig. 2

MenSCs induced T cell proliferation. MenSCs were either inactivated with Mitomycin C or remained intact. Some wells were also primed with IFN-γ prior to co-culture with T cells. CFSC-labeled purified CD4 + T cells were then co-cultured with MenSCs at different ratios and activated with T cell activation beads. The extent of T cell proliferation was then tested after five days by flow cytometry. The effect of BMSCs on T cell proliferation was also tested in the same conditions. Gating strategy is shown in panel (A). Representative flow cytometry dot plots and bar charts for MenSCs and BMSCs are shown in (B). T cells cultured in the absence of MenSCs or BMSCs served as biological control (BC). Data were expressed as mean ± SEM of 8 experiments. The signs (*), (**), (***), and (****) stand for p < 0.0332, p < 0.0021, p < 0.0002, and p < 0.0001, respectively

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