Fig. 6From: Endometrial mesenchymal stem/stromal cells: The Enigma to code messages for generation of functionally active regulatory T cellsIL-6, IL-10, and TGF-β were involved in MenSCs-mediated Treg generation. MenSCs treated with IL-1β and IFN-γ were co-cultured with CD4 + T lymphocytes at 1:8 ratio and activated with T cell activation beads. Different blockers were added to the co-cultures. After five days, percent of CD4 + CD25highFOXP3 + Tregs was quantified by flow cytometry (A, B). T cells cultured in the absence of MenSCs served as biological control (BC). The data obtained from each group were compared with biological control, and the results were displayed as mean ± SEM of 8 experiments. Functional activity of IDO in MenSCs was tested using a colorimetric assay (C). MenSCs were treated with IFN-γ and/or IL-1β for 48 h. IFN-γ-treated BMSCs served as positive control. Wells containing untreated cells or cells treated with Trp were considered as negative controls. Data were expressed as mean ± SEM of 16 different samples of MenSCs and BMSCs. The signs (*), (**), and (****) stand for p < 0.0332, p < 0.0021, and p < 0.0001, respectively. Indo: Indomethacin, 1-MT: 1-methyl tryptophan, Trp: TryptophanBack to article page