Fig. 3

Gal-9 elevates the immunoregulatory capacity of ERCs in inhibiting the proliferation of CD4⁺ and CD8⁺ T cells in AIH mice. 24 h after model induction, single-cell suspensions were made from spleens of different groups. Splenocytes and hepatocytes were stained with anti-mouse CD3 FITC, anti-mouse CD4 PE, anti-mouse CD8 Percp antibodies, and then analyzed by flow cytometry. A Representative dot plots depict the percentage of CD4⁺ T cell (CD3⁺CD4⁺). B Representative dot plots depict the percentage of CD8⁺ T cell (CD3⁺CD8⁺). C Immunohistochemical staining of CD4⁺ and CD8⁺ T cell infiltration in the liver. The black arrows show the positive staining areas. D, E Percentage of CD4⁺ T cells and CD8⁺ T cells in the spleen and liver. F, G CD4⁺ T and CD8⁺ T cells were quantified by using ImageJ software to identify and calculate the brown-black positive areas. The differences between groups were calculated using ANOVA. *p < 0.05, **p < 0.01